SULPHINDIGOTATE AGAR l8l 



Sulphindigotate Bouillon (Weyl). 



1. Measure out nutrient bouillon (vide page 163, sections i 

 to 6 1000 c.c.). 



2. Weigh out glucose, 20 grammes ( = 2 per cent.), sodium 

 sulphindigotate, i gramme ( = 0.1 per cent.), and dissolve in 

 the fluid. 



3. Tube, and sterilise as for bouillon. 



Sulphindigotate Gelatine (Weyl). 



1. Prepare nutrient gelatine (vide page 164, sections i to 7). 

 Measure out 1000 c.c. 



2. Weigh out glucose, 20 grammes ( = 2 per cent.), and sodium 

 sulphindigotate, i gramme ( = o . i per cent.) , and dissolve in 

 the hot gelatine. 



3. Filter through papier Chardin. 



4. Tube, and sterilise as for nutrient gelatine. 



Sulphindigotate Agar. 



1. Prepare nutrient agar (vide page 167, sections i to 8). 

 Measure out 1000 c.c. 



2. Weigh out glucose, 20 grammes ( = 2 per cent.), sodium 

 sulphindigotate, i gramme ( = o . i per cent.) , and dissolve in 

 the hot agar. 



3. Tube, and sterilise as for nutrient agar. 



NOTE. The Sulphindigotate media are of a blue colour, which 

 during the growth of anaerobic bacteria is oxidised and decolour- 

 ised to a light yellow. 



