INOSITE-FREE MEDIA. 183 



Media for the Study of the Chemical Composition of Bacteria. 

 Asparagin Medium (Uschinsky). 



1. Weigh out and mix 



Asparagin 3-4 grammes 



Ammonium lactate 10.0 grammes 



Sodium chloride 5.0 grammes 



Magnesium sulphate 0.2 gramme 



Calcium chloride o . i gramme 



Acid potassium phosphate (KH 2 PO 4 ) i . o gramme 



2. Dissolve the mixture in distilled water 1000 c.c. 



3. Add glycerine, 40 c.c. 



4. Tube, and sterilise as for nutrient bouillon. 



Asparagin Medium (Frankel and Voges). 



1. Weigh out and mix 



Asparagin 4 grammes 



Sodium phosphate, (Na 2 HPOj 1 2OH. . 2 grammes 



Ammonium lactate 6 grammes 



Sodium chloride 5 grammes 



and dissolve in 



Distilled water 1000 c.c. 



2. Tube, and sterilise as for nutrient bouillon. 



NOTE. Either of the above asparagin media, after the addition 

 of 10 per cent, gelatine or 1.5 per cent, agar, may be advan- 

 tageously employed in the solid condition. 



Proteid Free Broth (Uschinsky). 



1. Weigh out and mix 



Calcium chloride . . . . o . i gramme 



Magnesium sulphate . 0.2 gramme 



Acid potassium phosphate (KH 2 PO 4 ) . 2.0 grammes 



Potassium aspartate 3.0 grammes 



Sodium chloride 5.0 grammes 



Ammonium lactate .6.0 grammes 



2. Dissolve the mixture in distilled water 1000 c.c. 



3. Add glycerine 30 c.c. 



4. Tube and sterilise as for nutrient broth. 



Media for the Study of Bio-chemical Reaction. 



Inosite-free Media Bouillon (Durham). 



1. Prepare meat extract, 1000 c.c. (vide page 148), from 

 bullock's heart which has been "hung" for a couple of days. 



2. Prepare nutrient boullion (+10), 1000 c.c. (vide, page 161), 

 from the meat extract, and store in i -litre flask. 



