URINE GELATINE 187 



3. Weigh out and mix 



Asparagin 2 grammes 



Mannite 2 grammes 



and add to contents of flask. 



4. Measure out 25 c.c. of the solution and titrate it against 

 decinormal sodic hydrate, using litmus as the indicator. Control 

 the result and estimate the amount of sodic hydrate necessary 

 to be added to render the remainder of the solution neutral to 

 litmus. Add this quantity of sodic hydrate. 



5. Filter. 



6. Add litmus solution 47.5 c.c. ( = 5 per cent.). 



7. Tube, and sterilise as for nutrient bouillon. 



Capaldi=Proskauer Medium No. II. 



1. Weigh out and mix 



Peptone 20 grammes 



Mannite i gramme 



2. Dissolve in water 1000 c.c. in a 2-litre flask. 



3. Neutralise to litmus as in No. i (vide supra, Step 4). 



4. Filter. 



5. Add litmus solution 47.5 c.c. ( = 5 per cent.). 



6. Tube, and sterilise as for nutrient bouillon. 



Urine Media. Bouillon. 



1. Collect freshly passed urine in sterile flask. 



2. Place the flask in the steamer at 100 C. .for thirty minutes. 



3. Filter through two thicknesses of Swedish filter paper. 



4. Tube, and sterilise as for nutrient bouillon. 

 (Leave the reaction unaltered.) 



Urine Gelatine. 



1. Collect freshly passed urine in sterile flask. 



2. Take the specific gravity, and, if above lojo, dilute with 

 sterile water until that gravity is reached. 



3. Estimate (with control) at the boiling-point, and note the 

 reaction of the urine. 



4. Weigh out gelatine, 10 per cent., and add to the urine in 

 the flask. 



5. Heat in the steamer at 100 C. for one hour to dissolve the 

 gelatine. 



6. Estimate the reaction and add sufficient caustic soda 

 solution to restore the reaction of the medium mass to the 

 equivalent of the original urine. 



7. Cool to 60 C. and clarify with egg as for nutrient gelatine 

 (vide page 166). 



8. Filter through papier Chardin. 



9. Tube, and sterilise as for nutrient gelatine. 



