MILK RICE 189 



5. Tube in quantities of 10 c.c. and sterilise in the steamer at 

 100 C. for twenty minutes on each of three successive days. 



Lasvulose, galactose, maltose, lactose, etc., can be substituted 

 in similar amounts for dextrose and the medium completed as 

 above. 



Omeliansky's Nutrient Fluid (For Cellulose Fermenters) . 

 i. Weigh out and mix 



2. 



3. Flask in quantities of 250 c.c. 



4. Weigh out and add 5 grammes precipitated chalk to each 

 flask. 



5. Sterilise in the steamer at 100 C. for twenty minutes on 

 each of three successive days. 



Media for the Study of Chromogenic Bacteria. 



Milk Rice (Eisenberg). 



1. Measure out nutrient bouillon, 70 c.c., and milk, 210 c.c., 

 and mix thoroughly. 



2. Weigh out rice powder, 100 grammes, and rub it up in a 

 mortar with the milk and broth mixture. 



3. Fill the paste into sterile capsules, spreading it out so as 

 to form a layer about 0.5 cm. thick, over the bottom of each. 



4. Heat over a water-bath at 100 C. until the mixture 

 solidifies. 



5. Replace the lids of the capsules. Sterilise in the steamer 

 at 1 00 C. for thirty minutes on each of three consecutive days. 



(A solid medium of the colour of cafe au lait is thus produced.) 



Milk Rice (Soyka). 



1. Measure out nutrient bouillon, 50 c.c., and milk, 150 c.c., 

 and mix thoroughly. 



2. Weigh out rice powder, 100 grammes, and rub it up in a 

 mortar with the milk and broth mixture. 



3. Fill the paste into sterile capsules, to form a layer over the 

 bottom of each. 



4. Replace the lids of the capsules. 



5. Sterilise in the steamer at 100 C. for thirty minutes on 

 each of three consecutive days. 



(A pure white, opaque medium is thus formed.) 



