2O6 SPECIAL MEDIA 



5. Measure out and add to the nutrient agar, sodium sulphite, 

 10 per cent, aqueous solution, freshly prepared . . 25 c.c. 



6. Tube and sterilise as for nutrient agar. 



7. Store in a dark cupboard. 



Fuchsin Sulphite Agar (Endo). 



1. Liquefy and measure out into a sterile flask: 



Nutrient agar 1000 c.c. 



2. Weigh out 



Lactose 10 grammes. 



and dissolve in the fluid agar. 



3. Adjust the reaction to +3 and filter. 



4. Measure out and mix thoroughly with the fluid agar. 



Fuchsin, alcoholic solution (vide supra) .... 5 c.c. 



5. Measure out and add to the medium 



Sodium sulphite, 10 per cent, aqueous solution . .25 c.c. 



6. Tube and sterilise as for nutrient agar. 



Brilliant Green Agar (Conradi) . 



1 . Liquefy and measure out into a sterile flask 



Nutrient agar 1000 c.c. 



2. Adjust reaction to + 30 by the addition of normal phosphoric 

 acid ; and filter. 



3. Measure out and mix thoroughly with the fluid medium 



Brilliant green (Hoechst) i per thousand aque- 

 ous solution 6.5 c.c. 



4. Measure out and add to the medium 



Picric acid (Gruebler) , i per cent, aqueous solution . 6.5 c.c. 



5. Tube and sterilise as for nutrient agar. 



Brilliant Green Bile Salt Agar (Fawcus). 



1. Weigh out agar 20 grammes and emulsify in 100 c.c. cold 

 distilled water. 



2. Wash the emulsion into a "tared" 2-litre flask with 500 c.c. 

 distilled water. 



3 . Dissolve the agar by bubbling live steam through the flask. 



4. Cool, clarify with egg and filter. 



5. Weigh out 



Sodium taurocholate 5 grammes 



Peptone 20 grammes 



and add to the medium in the flask. 



