244 



METHODS OF CULTIVATION 



shaped bends in two lumps of plasterine stuck on the 

 bottom of the glass dish. Fix a short length of rubber 

 tubing clamped with a metal clip to each of the out- 

 side arms (Fig. 134). 



6. Fill the glass dish with glycerine or metallic 

 mercury to a depth of about 5 cm. 



FIG. 134. Botkin's apparatus. 



7. Connect up one U-shaped tube with the hydrogen 

 cylinder (or gas apparatus) by means of rubber tubing. 

 Replace the atmospheric air by hydrogen, as in method 

 IX. 



8. Clamp the tubes and disconnect the gas apparatus. 



9. Incubate. 



Method XI (Novy's Method) .- 



Apparatus Required. 



Jar for plate cultivations (Fig. 135). 

 Or, jar for tube cultivations (Fig. 136). 

 Lubricant for stopper of jar. 

 Rubber tubing. 

 Cylinder of compressed hydrogen. 



