ANAEROBIC CULTURES 



245 



METHOD. 



1. Prepare cultivations in the usual way. 



2. Place these inside the jar. 



3. Lubricate the stopper and insert it in the mouth 

 of the jar, with the handle in a line with the two side 

 tubes. 



4. Connect up the delivery tube a with the hydrogen 

 gas supply by means of rubber tubing. 



FIG. 135. Novy's jar for 

 plate cultivations. 



FIG. 136. Novy's jar for 

 tube cultivations. 



5. Attach a piece of rubber tubing to the exit tube 

 b and collect samples of the issuing gas (over water) 

 and test from time to time. 



6. When the air is completely displaced by hydrogen, 

 turn the handle of the stopper at right angles to the 

 line of entry and exit tubes; this seals the orifice of 

 both tubes. 



7. Disconnect the gas apparatus and incubate. 



(E) Method XII (Bulloch's Method) .- 



Apparatus Required. 



Bulloch's jar. 



Pot of resin ointment. 



Small glass dish 14 cm. diameter by 5 cm. deep. 



Vessel for tube cultures or metal rack for plate cultures. 



