260 METHODS OF IDENTIFICATION AND STUDY 



Agar plates, 



Agar streak or smear, 



Agar stab, 



Inspissated blood-serum, 



at2oC. and 3 7 C. 



Bouillon, 

 Litmus milk, 

 Potato, 



Special media for the purpose of demonstrating 

 characteristic appearances. 



2. Morphology. (Vide Microscopical Examination of 



Cultivations, page 272.) 

 Vegetative forms : 



Shape. 



Size. 



Motility. 



Flagella (if present) . 



Capsule (if present) . 



Involution forms. 



Pleomorphism (if observed) . 

 Sporing forms (if observed). Of which class? 

 Staining reactions. 



3. Chemical Products of Growth. (Vide Chemical 



Examination of Cultivations, page 276.) 

 Chromogenesis. 

 Photogenesis. 

 Enzyme formation. 



Fermentation of carbohydrates : 

 Acid formation. 

 Alkali formation. 

 Indol formation. 

 Phenol formation. 

 Reducing and oxidising substances. 

 Gas formation. 



4. Biology. ( Vide Physical Examination of Cultures, 



page 295.) 

 Atmosphere. 

 Temperature. 



