272 METHODS OF IDENTIFICATION AND STUDY 



Amount. 

 Colour. 



Carbohydrate Media. 



Growth. 

 Reaction. 

 Gas formation. 



Coagulation or not of serum albumen (when serum 

 water media are employed). 



Litmus Milk Cultivations. 



Unaltered. 



i. Reaction: 



Acid. 



Alkaline. 



2. Odour. 



3. Formation of gas. 



Unaltered. 



4. Consistency: 



Peptonised (character of solution) . 

 Coagulated. 



f hard: solid. 



soft : floculent. 

 Clot: Character -, -, , , , 



ragged and broken up by gas 



bubbles. 



(a) Coagulum undissolved. 



(b) Coagulum finally peptonised, completely : in- 



completely. 

 Resulting solution, clear: turbid. 



Abundant. 

 Scanty. 



6. Whey: Clear. 

 Turbid. 

 Coagulated by boiling, or not. 



BY MICROSCOPICAL METHODS. 



As a council of perfection preparations must be 

 made from pure cultivations 4, 6, 8, 12, 18, and 24 



