PREPARATION 335 



must be obtained in addition to the license; whilst if 

 the inoculation entails more extensive operative pro- 

 cedures, and it is necessary to observe the subsequent 

 course of the infection, should such occur, the license 

 must be coupled with Certificate B since this certifi- 

 cate removes the compulsion to destroy the animal 

 whilst under the anaesthetic. Further special certifi- 

 cates and combinations of certificates are required if 

 cats, dogs, horses, asses or cattle are to be the subjects 

 of experiment. Under every certificate it is expressly 

 stipulated that if the animal shows signs of pain it must 

 be destroyed immediately. 



The animals generally employed in the study of the 

 pathogenic properties of the various micro-organisms 

 are: 



Cold Blooded Warm Blooded. Hot Blooded. 



Frog. Mouse. Fowl. 



Toad. Rat. Pigeon. 



Lizard. Guinea pig. 



Rabbit. 



Monkey. 



Preparation. Before inoculation, the experimental 

 animals should be carefully examined, to avoid the 

 risk of employing such as are 'already diseased: since 

 it must be remembered that in a state of nature, as 

 well as in captivity, the animals employed for labora- 

 tory inoculations are subject to infection by various 

 animal and vegetable parasites, and in some instances 

 such infection presents no symptoms which are ob- 

 vious to the casual examination; the sex should be 

 noted, the weight recorded, and the rectal tempera- 

 ture taken. The remaining items of importance are 

 the time of the inoculation, the material that is inocu- 

 lated, and the method of inoculation, and finally under 

 what authority the experiment is performed. In the 

 author's laboratory these data are entered upon a pink 

 card which forms part of a card index system. The 



