344 EXPERIMENTAL INOCULATION OF ANIMALS 



As animal inoculation is purely a surgical operation, 

 the necessary instruments will be similar to those em- 

 ployed by the surgeon, and, like them, must be sterile. 

 In the performance of the inoculation strict attention 

 must be paid to asepsis, and suitable precautions 

 adopted to guard against accidental contamination of 

 the material to be introduced into the animal. In 

 addition, the hands of the operator should be care- 

 fully disinfected. 



The list of apparatus used in animal inoculations 

 given below comprises practically everything needed 

 for any inoculation. Needless to remark, all the appa- 

 ratus will never be required for any one inoculation. 



Apparatus Required for Animal Inoculation : 



i. Water steriliser (vide page 33) . It is also convenient to have 

 a second water steriliser, similar but smaller (23 by 7 by 5 cm.), 

 for the sterilisation of the syringes. 







FIG. 171. Hypodermic syringe with finger rests. 



2. Injection syringe. The best form is one of the ordinary 

 hypodermic pattern, i c.c. capacity graduated in twentieths of a 

 cubic centimeter (0.05 c.c.), fitted with finger rests, but with the 

 leather washers and the packing of the piston replaced by those 

 made of asbestos (Fig. 171). The instrument must be easily taken 

 to pieces, and spare parts should be kept on hand to replace acci- 

 dental breakage or loss. Other useful syringes are those of 2 c.c., 

 5 c.c., 10 c.c., and 20 c.c. capacity. A good supply of needles must 

 be kept on hand, both sharp-pointed and with blunt ends. To 

 sterilise the syringe, fill it with water, loosen the packing of the 

 piston and all the screw joints, place it in the steriliser and boil 

 for at least five minutes. Disinfect the syringe after use, in a 

 similar manner. The needles, which are exceedingly apt to rust 

 after being boiled, should be stored in a pot of absolute alcohol 

 when not in use. 



3. Operating table. 



4- Surgical instruments. Sterilise these before use by boiling, 

 and disinfect them after use by the same means. Wipe perfectly 

 dry immediately the disinfection is completed. 



