MATERIAL UTILISED FOR INOCULATION 



347 



(Or, remove a definite measured quantity of the cul- 

 tivation directly from the tube or flask by means of 

 a sterile graduated pipette, discharge the measured 

 amount into a sterile capsule, and fill into the syringe ; 

 or take up the required quantity of the cultivation 

 directly into the graduated syringe from the tube or 

 flask. - 



If it is necessary to introduce a large bulk of fluid 

 into the animal, the cultivation should be transferred 



FIG. 173. Conical separately funnel, fitted for injection of fluid cultivations. 



with aseptic precautions, to a sterile separatory funnel, 

 preferably of the shape shown in figure 173, and gradu- 

 ated if necessary. This is supported on a retort stand 

 and raised sufficiently above the level of the animal 

 to be injected, so as to secure a good " fall." A piece of 

 sterilised rubber tubing of suitable length, fitted with an 

 injection needle and provided with a screw clamp, is 

 now attached to the nozzle of the funnel and the opera- 



