METHODS OF SECURING ANIMALS 349 



(c) Toxins. Prepared by previously described 

 methods (vide page 318), are manipulated in a similar 

 manner to cultivations in fluid media. 



(d) Pathological Products. Fluid secretions, excre- 

 tions, etc., such as serous exudation, pus, blood, etc., 

 are treated as fluid cultivations ; but if the material is 

 very thick or viscous, a small quantity of sterile bouillon 

 or normal saline solution may be used to dilute it, and 

 thorough incorporation effected by the help of a sterile 

 platinum rod. 



Solid tissues, such as spleen, lymph glands, etc., 

 may be divided into small pieces by sterile instruments 

 and rubbed up in a sterilised agate mortar (using an 

 agate pestle, with a small quantity of sterile bouillon, 

 and the syringe filled from the resulting emulsion. 



FIG. 175. Holding rabbit for shaving. 



If it is desired to inoculate tissue en masse, remove 

 from the material a small cube of i or 2 mm. and 

 introduce it into a wound made by sterile instruments 

 in a suitable situation, and occlude the wound by 

 means of Michel's steel clips and a sealed dressing. 



Method of Securing Animals During Inoculation. 



For the majority of inoculations, especially when no 

 anaesthetic is administered, it is customary to employ 

 an assistant to hold the animal (see Fig. 175). 



If working single handed Voge's holder for guinea- 

 pigs, is a useful piece of apparatus the method of using 

 which is readily seen from the accompanying figures 

 (Figs. 176, 177). 



The instrument itself consists of a hollow copper 



