SUBCUTANEOUS 353 



selected for inoculation, with a wad of cotton- wool, 

 and lather freely by the aid of warm water; shave 

 carefully and thoroughly; or apply the depilatory 

 powder. 



3. Wash the denuded area of skin thoroughly with 

 2 per cent, lysol solution. 



4. Wash off the lysol with ether and allow the latter 

 to evaporate. 



5. Make numerous short, parallel, superficial inci- 

 sions with the point of a sterile scalpel. 



6. When the oozing from the incisions has ceased, 

 rub the inoculum into the scarifications by means of 

 the flat of a scalpel blade, or a sterile platinum spatula. 



7. Cover the inoculated area with a pad of sterile 

 gauze secured in situ by strips of adhesive plaster or 

 by sealing down the edges of the gauze with collodion. 



8. Release the animal, place it in its cage, and affix 

 a label upon which is written : 



(a) Distinctive name or number of the animal. 



(b) Its weight. 



(c) Particulars as to source and dose of inoculum. 



(d) Date of inoculation. 



2. Subcutaneous Inoculation. 



(a) Fluid Inoculum. (Anesthetic, none.) 

 Steps 1-4. As for cutaneous inoculation. 



5. Pinch up a fold of skin between the forefinger 

 and thumb of the left hand; take the charged hypo- 

 dermic syringe in the right hand, enter the needle into 

 a ridge of skin raised by the left finger and thumb, 

 and push it steadily onward until about 2 cm. of the 

 needle are lying in the subcutaneous tissue. Now 

 release the grasp of the left hand and slowly inject the 

 fluid contained in the syringe. 



6. Withdraw the needle, and at the same moment 

 close the puncture with a wad of cotton wool, to prevent 

 the escape of any of the inoculum. The injected fluid, 



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