354 EXPERIMENTAL INOCULATION OF ANIMALS 



unless large in amount, will be absorbed within a very 

 short time. 



7. Label, etc. 



(6) Solid Inoculum. (Anesthetic, none; or Ethyl 

 chloride spray.} 



Steps 1-4. As for cutaneous inoculation. 



5. Raise a small fold of skin in a pair of forceps, 

 and make a small incision through the skin with a 

 pair of sharp-pointed scissors or with the point of a 

 scalpel. 



6. Insert a probe through the opening and push it 

 steadily onward in the subcutaneous tissue, and by 

 lateral movements separate the skin from the under- 

 lying muscles to form a funnel-shaped pocket with its 

 apex toward the point of entrance. 



7. By means of a pair of fine-pointed forceps intro- 

 duce a small piece of the inoculum into this pocket 

 and deposit it as far as possible from the point of 

 entrance. 



FIG. 181. Glass tube syringe for subcutaneous "solid" inoculation. 



Or, improvise a syringe by sliding a piece of glass 

 rod (to serve as a piston) into the lumen of a slightly 

 shorter length of glass tubing and secure in position by 

 a band of rubber tubing. Sterilise by boiling. With- 

 draw the rod a few millimetres and deposit the piece of 

 tissue within the orifice of the tube, by means of sterile 

 forceps. Now pass the tube into the depths of the 

 "pocket,'* push on the glass rod till it projects beyond 

 the end of the tube, and withdraw the apparatus, 

 leaving the tissue behind in the wound. 



8. Close the wound in the skin with Michel's clips 

 and a dressing of gauze sealed with collodion (or Tinct. 

 benzoin) . 



9. Label, etc. 



