INTRAPERITONEAL 355 



3, Intramuscular. 



(a) Fluid Inoculum. (Ancesihetic, none.) 

 Steps 1-4. As for cutaneous inoculation. 



5. Steady the skin over the selected muscle or 

 muscles with the slightly separated left forefinger 

 and thumb. 



6. Thrust the needle of the injecting syringe boldly 

 into the muscular tissue and inject the inoculum 

 slowly. 



7. Label, etc. 



(6) Solid Inoculum. (Anesthetic, A. C. E.) 



1. Secure the animal to the operation table and 

 anaesthetise. 



2. Shave and disinfect the skin at the seat of opera- 

 tion. 



3 . Surround the field of operation by strips of gauze 

 wrung out in 2 per cent, lysol solution. 



4. Incise skin, aponeurosis, and muscle in turn. 



5. Deposit the inoculum in the depths of the incision. 



6. Close the wound in the muscle with buried sutures 

 and the cutaneous wound with either continuous or 

 interrupted sutures or with Michel's steel clips. 



7. Apply a sealed dressing of gauze and collodion. 



8. Remove the animal from the operating table. 



9. Label, etc. 



4. Intraperitoneal. 



(a) Fluid Inoculum. (Anesthetic, none.) 



Steps 1-4. As for cutaneous inoculation. Shave a 



fairly broad transverse area, stretching from flank 



to flank. 



5. Place the left forefinger on one flank and the 

 thumb on the opposite, and pinch up the entire thick- 

 ness of the abdominal parietes in a triangular fold. 

 Now, by slipping the peritoneal surfaces (which are in 

 apposition) one over the other, ascertain that no coils 

 of intestine are included in the fold. 



