356 EXPERIMENTAL INOCULATION OF ANIMALS 



6. Take the syringe in the right hand and with the 

 needle transfix the fold near its base (Fig. 182) . 



7. Now release the fold, but hold the syringe steady; 

 as the parietes flatten out, the point of the needle is 

 left free in the peritoneal cavity (see Fig. 183). 



FIG. 182. Intraperitoneal inoculation fluid. 



8. Inject the fluid from the syringe. 



9. Label, etc. 

 Second Method: 



Steps 1-4. As in the first method. 



5. Anaesthetise a small selected 

 area of skin by spraying it with 

 ethyl chloride. 



6. Heat platinum searing wire 

 (0.5 mm. wire, twisted to the 



FIG. 183. Section of ab- shape indicated in figure 184, 



dommal wall, etc., showing ., . <. . ^ . 



point of needle lying free mounted in an aluminum handle) 



t0 redneSS ' and With it bum a 

 hole through the anaesthetic area 



of skin and abdominal muscle down to, but not through, 

 the visceral peritoneum. 



7. Fix a blunt-ended needle on to the charged 

 syringe, and by pressing the rounded end firmly against 

 the peritoneum it can easily be pushed through into the 

 peritoneal cavity. 



8. Inject the fluid from the syringe. 



Ibo 



