COLLODION SACS 357 



9. Label, etc. 



This method is especially useful when it is desired 

 to collect samples of the peritoneal fluid from time to 

 time during the period of observation, as fluid can be 

 removed from the peritoneal cavity, at intervals, 

 through this aperture in the abdominal parietes, by 

 means of a sterile capillary pipette. 



FIG. 184. Platinum wire for burning hole through parietes. 



(6) Solid Inoculum (or the implantation of capsules 

 containing fluid cultivations). (Anesthetic, A. C. E.) 



1. Anaesthetise the animal and secure it to the 

 operating table. 



2. Shave a large area of the abdominal parietes. 



3. Make an incision through the skin in the middle 

 line about 2 cm. in length, midway between the lower 

 end of the sternum and the pubes. 



4. Divide the aponeuroses between the recti upon a 

 director. 



5. Divide the peritoneum upon a director. 



6. Introduce the inoculum into the peritoneal 

 cavity. 



7. Close the peritoneal cavity with Lembert's su- 

 tures. 



8. Close the skin and aponeurosis incisions together 

 with interrupted sutures or Michel's steel clips, and 

 apply a sealed dressing. 



9. Release the animal from the operating table. 



10. Label, etc. 



Suitable sacs may be readily prepared by either of 

 the following methods* 



A. Collodion Sacs. 



i. Dip a small test-tube (5 by 0.5 cm.), bottom 

 downward, into a beaker of collodion, and dry in the 

 air; repeat this process three or four times. 



