3 62 



EXPERIMENTAL INOCULATION OF ANIMALS 



7. Turn back the flap of skin and secure it in position 

 with Michel's steel clips. 



8. Dress with sterile gauze and wool and seal the 

 dressing with collodion. 



9. Label, etc. 



(b) Intracerebral. This inoculation is performed 

 precisely as for subdural save in step 

 6 the needle after perforating the dura 

 mater is pushed onward into the sub- 

 stance of one or other cerebral hemis- 

 pheres before the contents are ejected. 



6. Intraocular. 



(a) Fluid Inoculum. (Anesthetic, 

 cocaine.) 



1. Instil a few drops of a sterile solu- 

 tion of cocaine, and repeat the instilla- 

 tion in two minutes. 



2. Five minutes later have the animal 

 firmly held by an assistant as in intra- 

 venous injection (see Fig. 189) the head 

 being steadied by the assistant's hands. 



3. Select two needles to accurately fit 

 the same syringe and sterilise. 



4. Attach one needle to the syringe 

 and take up the required dose of inocu- 

 lum and remove the needle. 



5. Steady the eye with fixation forceps; then pierce 

 the cornea with the other syringe needle and allow the 

 aqueous to escape through the needle. 



6. Without removing the needle from the cornea 

 attach the syringe and make the injection into the 

 anterior chamber. 



7. Irrigate the conjunctival sac with sterile saline 

 solution. 



8. Label, etc. 



(b) Solid Inoculum. (Anesthetic, A. C. E.) 



FIG. 188. 

 tracranial inocula- 

 t i o n of rabbit. 



of the 

 hole. 



trephine 



