INHALATION 365 



root of the ear, and slowly inject the inoculum. (If 

 the fluid is being forced into the subcutaneous tissue, 

 a condition which is at once indicated by the swelling 

 that occurs, the injection must be stopped and another 

 attempt made at a spot closer to the root of the ear or 

 at some point on the corresponding vein on the opposite 

 ear.) - 



7 . Withdraw the needle and press a pledget of cotton- 

 wool over the puncture to ensure closure of the aperture 

 in the vein wall. 



8. Label, etc. 



FIG. 189. Intravenous inoculation. 



9. Inhalation. 



(a) Fluid Inoculum. (Anesthetic, none.) 



1. Place the animal in a closed metal box. 



2. Through a hole in one side introduce the nozzle 

 of some simple spraying apparatus, such as is used for 

 nasal medicaments. 



3. Fill the reservoir of the instrument (previously 

 sterilised) with the fluid inoculum, and having at- 

 tached the bellows, spray the inoculum into the interior 

 of the box. 



4. On the completion of the spraying, open the box, 

 spray the animal thoroughly with a 10 per cent, solution 

 of formaldehyde (to destroy any of the virus that may 

 be adhering to fur or feathers). 



5. Transfer the animal to its cage. 



