366 EXPERIMENTAL INOCULATION OF ANIMALS 



6. Label, etc. 



7. Thoroughly disinfect the inhalation chamber. 



(6) Fluid or Powdered Inoculum. A n&sthetic, A.C.E. 

 i. Anaesthetise the animal and secure it firmly to 

 the operating table. 



FIG. 190. Gag for rabbits. 



2. Prop open the mouth by means of some form of 

 gag ; seize the tongue with a pair of forceps and draw it 

 forward. 



The most convenient form of gag for the rabbit or 

 cat is that shown in Fig. 190. It is simply a strip of 

 hard wood shaped at the middle and provided with a 

 square orifice through which a tracheal or cesophageal 

 tube can be passed. 



3. Pass a previously sterilised glass tube (17 cm. 

 long, 0.5 cm. diameter, with its terminal 2 cm. slightly 

 curved) down through the larynx into the trachea. 



4. Connect the straight portion of a Y-shaped piece 

 of tubing to the upper end of the sterilised tube and 

 couple one branch of the Y to a separatory funnel con- 

 taining the fluid inoculum, or insufflator containing the 

 powdered inoculum, and the other to a hand bellows. 



5. Allow the fluid inoculum to run into the lungs by 

 gravity, or blow in the powdered inoculum by means 

 of a rubber-ball bellows. 



6. Remove the intratracheal tube ; release the animal 

 from the table. 



7. Label, etc. 



As an alternative method in the case of fairly large 

 animals, such as rabbits, etc., a sterile piece of glass 

 tubing of suitable diameter may be passed through 

 the larynx down the trachea almost to its bifurcation. 



