INTRAGASTRIC 367 



Fluid cultivations may then be literally poured into 

 the lungs, or cultivations, dried and powdered, may 

 be blown into the lung by the aid of a small hand 

 bellows or even a teat pipette. 



10. Intragastric Inoculation. Fluid or semifluid in- 

 oculum. (Anaesthetic none.) 



The method of performing the operation is varied 

 slightly according to the size of the experimental animal. 



A. Monkey, Rabbit, Guinea-pig. 



1. Secure the animal to the operating table ventral 

 surface uppermost. 



2 . Prop the mouth open with a gag ; draw the tongue 

 forward with forceps. 



3. Sterilise a soft rubber catheter (No. 10 or 8 Eng- 

 lish scale, or No. 1 8 or 15 French) and lubricate it with 

 sterile glycerine. 



4. Pass it to the back of the pharynx, keeping the 

 end in the middle line. 



5 . Gently assist the progress of the catheter down the 

 oesophagus until it passes the cardiac orifice of the 

 stomach. Do not use any force. 



6. Take up the required dose of inoculum into a 

 sterilised pipette. Insert the point of the pipette 

 into the open end of the catheter and allow the fluid 

 to run down into the stomach. Remove the pipette 

 and drop it into a jar of lysol. 



7. -With another sterile pipette run one cubic cen- 

 timetre of sterile saline solution through the catheter 

 to wash out the last, traces of the inoculum. 



8. Withdraw the catheter. 



9. Label, etc. 



B. Rats and Mice (Mark's Method). 



i. Secure the animal in the vertical position. 



(a) Rat. Take a pair of catch sinus forceps about 

 22 cm. in length and seize the animal by the loose skin 

 of the head as far forward as possible fix the forceps, 

 and holding the instrument vertically upward, transfer 



