FEEDING 369 



3 . Moisten the sterilised oesophageal tube with sterile 

 water. (This tube is of silk rubber, 6.5 cm. in length, 

 with the distal end rounded, the proximal end mounted 

 in a syringe needle head, which fits the nozzles of the 

 two sterile syringes to be used.) 



4. Grasp the tube about its middle and pass it into 

 the animal's mouth, downwards and a little to one side 

 or the other until its length is lost in the digestive tract 

 and mouth. Gentle guidance is alone necessary. Do 

 not use any force. 



5. Take up the required dose of inoculum into the 

 syringe ; insert the nozzle of the syringe into the needle- 

 mount, and force the piston down. 



6. Steadying the needle-mount with the left hand, 

 detach the syringe. 



7. Draw up some sterile water in the second (sterile) 

 syringe, and inserting its nozzle into the needle-mount 

 force a few drops of water through the tube to wash it 

 out. 



8. With one quick upward movement remove the 

 tube from the animal's mouth. 



9. Label, etc. 



One other method of inoculation remains to be 

 described, which does not require operative inter- 

 ference. 



11. Feeding. 



1. Fluid Inoculum. Small pieces of sterilised bread 

 or sop (sterilised in the steamer at 100 C.) are soaked 

 in the fluid inoculum and offered to the animals in a 

 sterile Petri dish or capsule. 



2. Solid Inoculum. Small pieces of tissue are placed 

 in sterile vessels and offered to the animals. 



