SPECIAL OBSERVATIONS 373 



Fluid secretions and excretions, such as pus or 

 serous exudates when accessible are collected direct 

 from the body in sterile capillary pipettes (vide Fig. 

 130,) in the following manner: 



i. Open the case containing the pipettes, grasp one 

 by the plugged end, remove it from the case, and 

 replace the lid of the latter. 



2. Attach a rubber teat (vide page 10) to the plugged 

 end of the pipette and use the teat as the handle of the 

 pipette. 



3. Pass the entire length of the pipette twice or 

 thrice through the flame of the Bunsen burner. 



4. Snap off the sealed end of the pipette with a pair 

 of sterile forceps. 



5. Compress the india-rubber teat, thrust the point 

 of the pipette into the secretion ; now relax the pressure 

 on the teat and allow the pipette to fill. 



6. Remove the point of the pipette from the secretion, 

 allow the fluid to run a short distance up the capillary 

 stem and seal the point of the pipette in the flame. (If 

 using a pipette with a constriction below the plugged 

 mouthpiece (Fig 136), this portion of the pipette may 

 also be sealed in the flame.) 



When ready to examine the morbid material snap 

 off the sealed end of the pipette with sterile forceps and 

 eject the contents of the pipette into a sterile capsule. 

 The material can now be utilized for coverslip prep- 

 arations, cultivations and inoculation experiment. 



3. The peripheral blood should be examined from 

 time to time for from this tissue is often obtained 

 the fullest information as to the course and progress 

 of an infection. 



a. The histological examination of the blood should 

 be directed chiefly to observations on the number and 

 kind of white cells; and since but few bacteriologists 

 are at the same time expert comparative haematolo- 

 gists, some notes on the normal characters of the blood 



