PERITONEAL CAVITY 399 



13. Grasp the apex of the heart in the forceps and 

 sear the surface of the right ventricle. 



14. Plunge the open point of a capillary pipette 

 through the seared area into the ventricle and fill with 

 blood. 



Make cultivations and cover-slip preparations of the 

 heart blood. 



15. Collect a further sample of blood or serum for 

 subsequent investigation as to the presence of anti- 

 bodies. 



Peritoneal Cavity. 



1 6. Sear a broad track in the middle line of the abdom- 

 inal wall; open the peritoneal cavity by an incision 

 in the centre of the seared line. Observe the condition 

 of the omentum, the mesentery, the viscera and the 

 peritoneal surface of the intestines. 



17. Collect a specimen of the peritoneal fluid (or pus, 

 if present) in a capillary pipette. Make cultivations, 

 tube and surface plate, and cover-slip preparations 

 from this situation. 



1 8 . Collect a specimen of the urine from the distended 

 bladder in a large pipette (in the manner indicated for 

 heart blood) , for further examination, by cultivations, 

 microscopical preparations, and chemical analysis. 



19. Collect a specimen of bile from the gall bladder 

 in similar manner. 



20. Excise the spleen and place it in a sterile cap- 

 sule. Later, sear the surface of this organ; plunge the 

 spear-headed spatula through the centre of the seared 

 area, twist is round between the finger .and thumb, and 

 remove it from the organ. Sufficient material will be 

 brought away in the eye in its head to make cultiva- 

 tions. A repetition of the process will afford material 

 for cover-slip preparations. 



21. Seize one end of the spleen with sterile forceps. 

 Sear a narrow band of tissue, right around the organ 



