400 POST-MORTEM EXAMINATIONS 



and divide the spleen in this situation with a pair of 

 scissors. Holding the piece of spleen in the forceps, 

 dab the cut surface on to a surface plate in a number of 

 different spots. 



22. In like manner examine the other organs liver, 

 lungs, kidneys, lymphatic glands (mesenteric, hepatic, 

 lumbar, etc), etc. Prepare cultivations and cover-slip 

 preparations. 



23. Dissect out a long bone from one upper and 

 one lower limb and one of the largest ribs. Prepare 

 cultures from the bone marrow in each case. Set aside 

 these bones for the subsequent preparation of marrow 

 films. 



24. Film preparations of bone marrow are best made 

 by the Price- Jones method. Seize the bone in a pair 

 of pliers and squeeze out some of the marrow ; receive 

 it in a platinum loop, and transfer to a watch glass of 

 dissociating fluid and emulsify. The dissociating fluid 

 is a neutral 10 per cent, solution of glycerine prepared 

 as follows : 



* 



Measure out 10 c.c. Price's best glycerine and 90 c.c. sterile 

 ammonia-free distilled water. Mix. Titrate against sodic hy- 

 drate solution using phenolphthalein as the indicator. The initial 

 reaction is usually + o.i to + 0.5; add the calculated amount of 

 sodic hydrate solution to neutralise. 



25. Place a loopful of fresh desiccating fluid on a 

 3X1 glass slide ; add a similar loopful of the marrow 

 emulsion, and spread very gently over the surface of 

 the slip. 



26. Allow film to dry in the air (protected from 

 dust) without heating. 



27. Stain with Jenner's polychrome stain (page 97) 

 for two and a half minutes. 



28. Wash with ammonia-free distilled water, dry 

 thoroughly and mount in xylol balsam. 



