STUDY OF PATHOGENIC BACTERIA 411 



abscess formation by Staphylococcus aureus) by 

 Gram's method, and counterstain with eosin. 



6. Stain film preparation of pus from an abscess 

 (containing Staphylococcus pyogenes aureus) with 

 carbolic methylene-blue and also by Gram's method, 

 counterstained with eosin. 



7. Inoculate 1 a white mouse subcutaneously with 

 three loopfuls of a forty-eight-hour agar cultivation of 

 the Staphylococcus aureus, emulsified with 0.2 c.c. 

 sterile broth. 



Observe carefully during life, and when death occurs 

 make a careful post-mortem examination. 

 II. Pyo genie cocci. 



Micrococcus gonorrhoea?. 



Micrococcus intracellularis meningitidis 



(meningococcus) . 

 Micrococcus catarrhalis. 

 Micrococcus tetragenus. 

 Micrococcus paratetragenus. 



III. Pyo genie cocci. 



Streptococcus pyogenes longus. 

 Streptococcus of bovine mastitis. 

 Streptococcus lanceolatus (Diplococcus 



pneumonise or pneumococcus) . 



to contrast with 

 Streptococcus brevis. 

 Streptococcus lebensis. 



IV. Pyo genie bacilli. 



Bacillus pneumonias (Friedlaender) . 

 Bacillus rhinoscleromatis. 

 Bacillus lactis aerogenes. 



V. Pyogenic bacilli. 



Bacillus pyocyaneus. 



to contrast with 



Bacillus fluorescens liquefaciens. 

 Bacillus fluorescens non -liquefaciens. 



1 See note on Vivisection License, page 334. 



