MILK 441 



7. Pick off any colonies resembling those of anthrax 

 and subcultivate on all the ordinary laboratory media. 



8. Inoculate another young white rat as in 3, using 

 two loopfuls of the agar subcultivation emulsified with 

 i c.c. sterile bouillon. Observe during life, and if the 

 animal succumbs, make a complete post-mortem 

 examination. 



B. TetanL 



1. Proceed as detailed above in steps i and 2 for 

 the isolation of the B. anthracis. 



2. Add i c.c. of the suspension to each of three tubes 

 of glucose formate broth, and incubate anaerobically 

 in Buchner's tubes at 3 7 C. 



3. From such of the tubes as show visible growth 

 (with or without the production of gas) after twenty- 

 four hours' incubation inoculate guinea-pigs, subcu- 

 taneously (under the skin of the abdomen), using o.i 

 c.c. of the bouillon cultivation as a dose. Observe 

 carefully during life, and, if death occurs, make a 

 complete post-mortem examination. 



4. From the same tubes pour agar plates and in- 

 cubate anaerobically in Bulloch's apparatus, at 37 C. 



5. Subcultivate suspicious colonies on the various 

 media, incubate anaerobically, making control cultiva- 

 tions on glucose formate agar, stab and streak, to 

 incubate aerobically and carry out further inoculation 

 experiments with the resulting growths. 



EXAMINATION OF MILK. 



"One-cow" or "whole" milk, if taken from the 

 apparently healthy animal (that is, an animal without 

 any obvious lesion of the udder or teats) with ordinary 

 precautions as to cleanliness, avoidance of dust, etc., 

 contains but few organisms. In dealing with one-cow 

 milk, from a suspected, or an obviously diseased animal, 

 a complete analysis should include the examination 

 (both qualitative and quantitative) of samples of 



