MILK 453 



bouillon cultivation derived from the first subcultiva- 

 tion of each glucose fermenter, and observe the result. 



6. If death, apparently from diphtheritic toxaemia, 

 ensues, inoculate two more guinea pigs with a similar 

 quantity of the lethal culture. Reserve one animal 

 as a control and into the other inject 1000 units of 

 antidiphtheritic serum. If the control dies and the 

 treated animal survives, the proof of the identity of 

 the organism isolated with the Klebs-LoefHer bacillus 

 becomes absolute. 



7. Inoculate guinea-pigs subcutaneously with filtered 

 glucose bouillon cultivations (toxins ?) and observe 

 the result. 



(B) i. Emulsify the remainder of the deposit with 



5 c.c. sterile bouillon and inoculate two guinea-pigs, 

 thus: guinea-pig a, subcutaneously with i c.c. emul- 

 sion; guinea-pig b, subcutaneously with 2 c.c. emulsion; 

 and observe the result. 



2. If either or both of the inoculated animals suc- 

 cumb, make complete post-mortem examination and 

 endeavour to isolate the pathogenic organisms from 

 the local lesion. Confirm their identity as in A 5 and 



6 (vide supra). 



5. Bacillus Tuberculosis. 



(A) i. Inoculate each of three guinea-pigs (previ- 

 ously tested with tuberculin, to prove their freedom 

 from spontaneous tuberculosis) subcutaneously at the 

 inner aspect of the bend of the left knee, with i c.c. 

 of the deposit emulsion remaining in one or other 

 tube D'or D 2 ). 



2. Introduce a small quantity of the cream into a 

 subcutaneous pocket prepared at the inner aspect of 

 the bend of the right knee of each of these three ani- 

 mals. Place a sealed dressing on the wound. 



3. Observe carefully, and weigh accurately each day. 



4. Kill one guinea-pig at the end of the second 



