462 BACTERIOLOGICAL ANALYSES 



10. Subcultivate from the colonies that make their 

 appearance and identify the various organisms. 



1 1 . Continue the investigations with reference to the 

 detection of pathogenic organisms as described under 

 water (page 429 et seq.). 



Qualitative. 



I. Cultural. 



The micro-organisms sought for during the examina- 

 tion of unsound foods comprise the following : 



Members of the Coli-typhoid groups (chiefly those of 

 the Gaertner class). 



B. anthracis. 



Streptococci 



Anaerobic Bacteria : 



B. enteritidis sporogenes. 

 B. botulinus. 

 B. cadayeris. 



The methods by which these organisms if present 

 may be identified and isolated have already been 

 discribed under the corresponding section of water 

 examination with the exception of those applicable to 

 B. botulinus, and B. cadaveris. These can only be 

 isolated satisfactorily from the bodies of experimen- 

 tally inoculated animals. 



II Experimental 

 Tissue. 



1. Feed rats and mice on portions of the sample 

 and observe the result. 



2. If any of the animals die, make complete post- 

 mortem examinations and endeavour to isolate the 

 pathogenic organisms. 



