466 BACTERIOLOGICAL ANALYSES 



oughly. Carry over i c.c. of fluid from capsule II to 

 capsule III, afterwards adding i c.c. of fluid from capsule 

 III to capsule IV. 



15. Label tubes of bile salt broth and inoculate with 

 the following amounts of diluted oysters : 



No. 6 with 10 c.c. cylinder fluid =0.1 oyster. 



No. 5 with i c.c. cylinder fluid = 0.01 oyster. 



No. 4 with i c.c. capsule I fluid = 0.001 oyster. 



No. 3 with i c.c. capsule II fluid = 0.0001 oyster. 



No. 2 with i c.c. capsule III fluid = 0.00001 oyster. 



No. i with i c.c. capsule IV fluid = 0.000001 oyster. 



1 6. Transfer 100 c.c. cylinder fluid (= i oyster) to an 

 Erlenmeyer flask and add 50 c.c. double strength bile 

 salt broth, and label 7. 



17. Duplicate all the above indicated cultures. 



18. Put up the tube cultures in Buchner's tubes and 

 incubate anaerobically at 42 C. 



If growth occurs in tube I the organism finally iso- 

 lated, e.g., B. coli, must have been present to the extent 

 of one million per oyster. 



19. Complete the examination for members of the 

 Coli-typhoid group and sewage streptococci, as directed 

 under Water Examination, page 429 (steps 11-21). 



20. Inoculate a series of 6 tubes of litmus milk with 

 quantities of the material similar to those indicated in 

 step 1 5 ; heat to 80 C. for ten minutes, and incu- 

 bate under anaerobic conditions at 37 C. Examine 

 for the presence of B. enteritidis sporogenes as directed 

 under Water Examination, page 438 (steps 7-10). 



EXAMINATION OF SEWAGE AND SEWAGE EFFLUENTS. 



Quantitative. 



Collection of the Sample. As only small quantities 

 of material are needed, the samples should be collected 

 in a manner similar to that described under water for 



