SOIL 477 



bouillon (technique similar to that described for the 

 water sample, vide pages 434-436). 



Every cubic centimetre of suspension now contains 

 the soil water from nearly i gramme of earth. 



The methods up to this point are identical no matter 

 which organism or group of organisms it is desired 

 to isolate; but from this stage onward the process is 

 varied slightly for each particular bacterium. 



I. The Coli=typhoid Group. 



II. Streptococci. 



III. Bacillus Anthracis. 



IV. Bacillus TetanL 



The methods adopted for the isolation of these 

 organisms are identical with those already described 

 under water (page 43 7 et seq.) . 



V. Bacillus (Edematis Maligni. Method precisely 

 similar to that employed for the B. tetani. 



VI. The Nitrous Organisms. 



1. Take ten tubes of Winogradsky's solution No I 

 (vide page 198) and number them consecutively from 

 i to 10. 



2. Inoculate each tube with varying quantities of 

 the material as follows : 



To tube No. i add i . o c.c. of the soil water. 



To tube No. 2 add o . i c.c. of the soil water. 



To tube No. 3 add i.o c.c. from Capsule I. 



To tube No. 4 add o. i c.c. from Capsule I. 



To tube No. 5 add i . o c.c. from Capsule II. 



To tube No. 6 add o. i c.c. from Capsule II. 



To tube No. 7 add i.o c.c. from Capsule III. 



To tube No. 8 add o.i c.c. from Capsule III. 



To tube No. 9 add i .o c.c. from Capsule IV. 



To tube No. 10 add o. i c.c. from Capsule IV. 



Label and incubate at 30 C. 



