APPENDIX 503 



7. Employ one portion of the final deposit to inoc- 

 ulate guinea pigs. 



8. Plant the remainder of the deposit freely on 

 Dorset's Egg medium; cap and incubate at 37 C. 



NOTE.. If only microscopical films are needed, fill up the centri- 

 fuge tube with Ligroin (a petroleum ether) in place of sterile dis- 

 tilled water in step 5 and prepare the films from the surface of the 

 fluid to stain by the Ziehl-Neelsen process. 



