LABORATORY STUDIES AND DEMONSTRATIONS. 22^ 



Collodion and Clove-oil. — Used for fixiiiij: sections to the slide 

 in order to prevent the displacement of deUcate or isolated part& 

 in balsam-monnting. Mix one part of ether-coll()di<»n and tliree 

 parts of oil of cloves. In niountiiii^, varnish a sHde with the 

 mixture by means of a camel' s-hair brush, lay on tlie sections, 

 and place the slide for a few minutes on the water-hath (i.e., 

 until tlie clove-oil evaporates). Transfer the slide trj a wide- 

 mouthed bottle of turpentine (to dissolve tlie ])arathn), remove it 

 and drain off the turpentine, place a drop of Canada balsam on 

 the middle of a cover-glass, and invert it over the object. 



Dahlia. — Dissolve in water. 



Eosin. — Dissolve in water until a bright-red solution is ob- 

 tained. It should be diluted when used. 



Glycerine, dilute. — Two parts glycerine, one part distilled 

 water. 



Haematoxylin (Delafield's). — Add 4 c.c. of saturated alcoholie 

 solution of hgematoxylin to 150 c.c. of strong aqueous solution of 

 anmionia-alum ; let the mixture stand a week or more in the 

 light, filter, and add 25 c.c. of glycerine and 25 c.c. of methyl 

 alcohol. The fluid improves greatly after standing some weeks 

 or months. 



Haematoxylin (Kleinenberg^s). — To a saturated solution of cal- 

 cium chloride in 70^ alcohol add an excess of pure alum ; filter 

 after 24 hours and add 8 volumes of 70^ alcohol, filtering again 

 if necessarv. Add a saturated alcoholic solution of lueniatoxylin 

 until the liquid becomes purple- blue. The longer the liquid 

 stands before using, the better. It should be diluted for use 

 with the alum-calcium-chloride solution in 70,^^ alcohol. 



Hermann's Fluid. — See Lee's Vade Mecum. 



Iodine Solution. — Dissolve jiotassium iodide in a small quantity 

 of water, add metallic iodine until the mixture assumes a dark- 

 brown color, and then dilute to a dark-sherry color. The solu- 

 tion should be kept from the light. 



Magenta (Aniline Red). — Dissolve in water. 



Methyl Green. — Used in aqueous or alcoholic solution or 



with acetic acid. 



Normal Fluid (Normal Salt Solution). — Dissolve 7.50 grams of 

 sodium chloride in 1 litre of distilled water. 



Paraffin.— "Hard" and "soft" paraffins, i.e., those of high 



