Agar 

 Hanging-block 



BACTERIOLOGICAL TECHNIC IO Q 



Orientation (grouping) 



Chains (No. of elements) 



Short chains, long chains. 



Orientation of chains, parallel, irregular. 



2. Sporangia, medium used temp age days 



Form, elliptical, short rods, spindled, clavate, drum-sticks. 

 Limits of Size Size of Majority 



(Orientation (grouping) 

 Chains (No. of elements) 

 Orientation of Chains, parallel, irregular. 

 Location of Endospores, central, polar. 



3. Endospores. 



Form, round, elliptical, elongated. 



Limits of Size 



Size of Majority 



Wall, thick, thin. 



Sporangium wall, adherent, not adherent. 

 Germination, equatorial, oblique, polar, bipolar, by stretching. 

 4- Flagella, No.. Attachment, polar, bipolar, peritrichiate. How Stained 



5. Capsules, present on 



6. Zooglea, Pseudozooglea. 



7. Involution Forms, on in days at C. 



8. Staining Reactions. 



i :io watery fuchsin, gentian-violet, carbol-fuchsin. Loeffler's alkaline methylene 



blue. 

 Special Stains 



Gram Glycogen 



Fat Acid-fast. 



Neisser 



II. CULTURAL FEATURES (3) 



1. A gar Stroke. 



Growth, invisible, scanty, moderate, abundant. 



Form of growth, filiform, echinulate, beaded, spreading, plumose, arborescent, rhizoid. 



Elevation of growth, flat, effuse, raised, convex. 



Luster, glistening, dull, cretaceous. 



Topography, smooth, contoured, rugose, verrucose. 



Optical Characters, opaque, translucent, opalescent, iridescent. 



Chromogenesis (8) 



Odor, absent, decided, resembling 



Consistency, slimy, buytrous, viscid, membranous, coriaceous, brittle. 

 Medium grayed, browned, reddened, blued, greened. 



2. Potato. 



Growth, scanty, moderate, abundant, transient, peristent. 



Form of growth, filiform, echinulate, beaded, spreading, plumose, arborescent, rhizoid. 



Elevation of/growth, flat, effuse, raised, convex. 



Luster, glistening, dull, cretaceous. 



Topogta.phy, f smooth, contoured, rugose, verrucose. 



Chromogenesis (8) Pigment in water insoluble, soluble; other 



solvents. . 



