4I2 PHARMACEUTICAL BACTERIOLOGY 



Bacteria P er cc - 



Mold (hyphal fragments) ... per cc. 



Mold spores per cc. 



Smut spores P er cc - 



Conclusions 



Analyst. 



We may give an example of a report as follows: 



FORM No. II 

 Lab. No. 462. 



Label: Pure currant jelly. Made by Smith, Jones & Co., Nantucket, Wis. 

 Sample received August 5, 1914. Sample examined August 5, 1914. 

 Condition of seals : Good, unbroken sample. 

 Organoleptic tests: Not conclusive. 



Consistency or feel: Poorly jellied. 

 Color: Normal for Currant jetty. 

 Odor: Faint, somewhat disagreeable. 

 Taste : Not characteristic, bitterish, quite acid. 

 Adjunct tests. 



Sublimation tests for 



Benzoicacid: Negative. 

 Salicylic acid : Very marked. 

 Boric acid (curcuma thread) : Negative. 

 Iodine reaction : Very marked. 

 Intracellular: Negative. 

 Extracellular: Positive, very marked. 



Special tests : Salicylic acid color reaction, with ferric chloride very marked. 

 Microscopical examination. 



General. Some apple tissue (window cells and pulp cells') and currant tissue 



sclerenchyma present. Added wheat starch about 5 per cent. 

 Cytometric counts. 



Dead yeast cells, 80,000,000 per cc. 



Living yeast cells, none per cc. 



Bacteria, 600,000,000 per cc. 



Mold (hyphal fragments and clusters), 84,000 per cc. 



Mold spores, 5,000,000 per cc. 



Smut spores, none per cc. 



Conclusions: Misbranded. Adulterated with apple and with wheat starch and made 

 from fermented and decomposed material, preserved with salicylic acid. Not fit for 

 human consumption because of the quantity of yeast, mold and bacteria present. 



JOHN DOE, Analyst. 



Course II. Bacteriological Quantitative and Qualitative Determi- 

 nations of Organisms in Foods and Drugs. 



A course in general laboratory technic is the necessary preparation 

 to this course. Such preparatory course should be given during the 

 third year of the full four year course, which would mean that the 

 present course must be given during the fourth year. 



