24 



conductivity of one -half is chiefly due to chlorides detached in 

 the process of dialysis. It is only after incineration and making 

 up to original volume that the conductivities of corpuscles and 

 serum become practically equal. 



The following table illustrates these interesting changes in 

 conductivity accompanying detachment of colloid and crystalloid 

 in two experiments on separated blood corpuscles and serum. 

 The figures give specific conductivity multiplied by 10 6 to save 



decimals. 



SAMPLE I. SAMPLE II. 



Treatment to which subjected. Serum. Corpuscles. Serum. Corpuscles. 



1. Fresh 170.") 95 1519 109 



2. Frozen solid ;md thawed K fif)9 1Q 14fifl ,- 



(corpuscles laked) . . | 1( 



3. Dialysed and volume re-) 1843 8yi 1623 



duced to original . . > 



4. Incinerated and ash made > 1G08 167? 169? 



up to original volume . ) 



That the phosphates are more firmly held than the chlorides, 

 so that the union persists even in presence of a very low concen- 

 tration of phosphatic ions in the fluid, is shown by the following 

 analysis for chlorides and phosphates in the dialysates of corpuscles 

 and serum respectively, and in the two cases after incineration. 

 The figures also illustrate the very different distribution of chlorides 

 and phosphates in corpuscles and serum respectively. 



Serum Percentages. Corpuscle Percentages. 



Cl. P;.O 5 . Cl. PjO 6 . 



Dialysis . . . 0*3657 0'0197 01331 0'0329 



Incineration . . 0'3:373 0'0219 01704 0'1708 



Even the chlorides are more strongly held in corpuscles than in 

 serum, the figure on incineration being considerably higher than 

 after dialysis, 0-1704 as against 0-1331, instead of being slightly 

 lower, due to volatilisation with organic matter, as in the case of 

 the serum, 0-3373 as against 0-3657. In the serum the phosphate 

 figures are almost equal by the two methods, but in the corpuscles 

 the evidence of union is clear, only 0-0329 per cent, dialyses out of 

 the 0-1708 shown to be present by incineration. These figures are 

 completely confirmed by freezing point determinations. 



This experimental evidence is interesting as showing that the 

 special affinities existing in each case between protein and ion 



