8 MAKING FIXED FILMS 



only fixed at the periphery the ring being the 

 line up to which the osmic acid has succeeded 

 in penetrating. The outer portions of the cover- 

 glass, therefore, should he the part examined, 

 when it will he found that the cells are nicely 

 spread out and that many of the lymphocytes 

 show some aspect of division figures. (See 

 illustrations.) 



Of course, by no means all the white cells 

 adhere to the cover-glass, many being left on the 

 jelly film ; and therefore several fixed films have 

 to be made before many dividing cells can be 

 found. In any case, in our opinion, the pictures 

 presented by these fixed preparations do not equal 

 the staining of the living cells as previously 

 described (1). 



