54 RED BLOOD-CORPUSCLES 



divisions in red cells by means of a solution of 

 haemoglobin which had been rendered thermostable 

 by the action of pancreatin ; then it was noticed 

 that, although the jelly containing the filtrate of 

 such haemoglobin (globin ?) induced no change in 

 the cells of healthy blood, the granular cells from 

 a case of secondary anaemia at once began to 

 divide when a high concentration of stain was 

 added to the jelly. 



This jelly was prepared as follows : 5 c.c. of a 

 2-per-cent solution of agar in water boiled and 

 filtered. To this was added 1 c.c. of normal saline 

 solution and 0'3 c.c. of a 5-per-cent solution of 

 sodium bicarbonate in water. A solution of blood 

 was made in a test-tube consisting of equal parts 

 blood and water. The corpuscles were laked and the 

 haemoglobin set free in the water. To this was 

 added a gramme of pancreatin powder (Pairchild) 

 and the test-tube kept at blood heat for twenty- 

 four hours. The solution of haemoglobin was then 

 filtered, and the filtrate boiled and filtered again. 

 1 c.c. of this second filtrate was added to the jelly 

 with 1 c.c. of azur II stain in a 2-per-cent solu- 

 tion. The whole jelly was then made up to a 

 volume of 10 c.c. with water, and when molten a 

 drop was poured on to a microscope slide and 

 allowed to set in a thin film. A drop of blood 

 from a healthy mammal was placed on a cover- 

 slip and dropped on the surface of the set jelly, 

 and the slide incubated for ten minutes. When 

 examined under the microscope the cells were 

 well spread out. Many of the leucocytes were 

 burst, while others had their granules stained 

 a deep blue. In many of the leucocytes also 



