58 RED BLOOD-CORPUSCLES 



What appears to be the centrosome having been 

 recognised by this method of demonstration, it 

 remained to show it by the fixed-film method 

 of observation. A very anaemic blood was chosen 

 one taken from an animal dying of trypano- 

 somiasis and this was stained carefully by 

 Giemsa's method. In some of those cells which 

 showed the punctate basophilia (granular erythro- 

 cytes) a minute red-staining dot could be dis- 

 tinguished which coincided with the centrosomes 

 seen during division induced in these erythro- 

 blasts by the jelly method. These centrosomes 

 when stained in anaemic-blood fixed films and 

 they can be found in any form of extreme 

 anaemia must bear a great resemblance to those 

 chromatic points described variously by authors 

 as solitary parasites, or infective granules, or arte- 

 facts. But by the jelly method they are readily 

 distinguished. Sometimes if the index of diffusion 

 of the jelly is high, e.g. when there is much 

 more alkali solution in it, or when it contains 

 a little 1-per-cent morphia solution, a condition 

 of vacuolation is induced in lymphocytes, leuco- 

 cytes, blood-plates, and erythroblasts. If stain 

 is present in the jelly these vacuoles stain red 

 like the centrosomes ; but if the vacuoles are 

 watched they grow in size and numbers, and can 

 thus be readily differentiated from the true centro- 

 somes ; and as the cells die and the cytoplasm 

 liquefies such vacuoles burst. 



The number of granules passing into each of 

 the daughter cells is dependent upon the rapidity 

 with which the process of division is produced ; 

 this again depends upon the amount of auxetic 



