46 



THE VEGETABLE CELL IN GENERAL. 



protein crystal-like bodies, and the protein basis or stroma in 

 which all of these are held. 



The protein basis sometimes, if not alwa3 - s, appears to consist 

 of two substances, differing in their solubility in water, and com- 

 mingled as granulose and 

 cellulose are in starch- 

 granules. While the pro- 

 tein basis is generally very 

 c soluble in water (not per 



se, but owing to the pres- 

 ence of potassic phos- 

 phate), the protein crystals are insoluble, or only slightly affected 

 by it, usuall}' becoming more or less swollen. After solution of 

 the protein basis has taken place, a delicate membrane is left 

 behind, and through this transparent film the protein crystals 

 are clearly seen. The relative amounts of protein basis and 

 protein crystals vary widehy ; in some cases the former appears 

 to be wanting, the latter wholly filling the interior of the mem- 

 brane. Such crystals appear in potato-tubers in the form of 



small cubes. Protein crystals of great beauty are easily dem- 

 onstrated in the endosperm of the common Brazil-nut (Ber- 

 tholletia). Very instructive phenomena are presented when 

 different sections of the seed are subjected to the following 

 reagents; (1) osmic acid (one per cent solution) ; (2) hteniatoxylin 



FIG. 14. Single protein granules treated as In Fig. 12. ?". (Pfeffer.) 



FIG. 15. Protein granules from Silybtim marlanum. In the cell on the left they have 

 crystalline contents; In that on the right, globolds. This section was taken from the 

 cotyledons of a dormant seed, and after treatment with mercuric chloride in alcohol was 

 placed in water. p>. (Pfeffer.) 



FIG. 16. The mesh of the ground mass of the cell has been cleared by dilute potassic 

 hydrate and hydrochloric m-id. n = nucleus. ?. (Pfeffer.) 



FIG 17. Cells from the cotyledons of a germinating seed which has just ruptured the 

 seed-coat. The protein granules have disappeared, but their contents are recognizable. 

 ?". (Pfeffer.) 



FIG 18. Stlybum marlanum Cell from the cotyledon of a nearly ripe seed In which 

 the formation of protein granules has just begun. ','". (Pfeffer.) 



