18 THE ELEMENTS OF VEGETABLE HISTOLOGY 



7. Sectioning. 



(a) Fixation of specimen on slide, if necessary. 



8. Removal of infiltration medium. 



(a) Mounting in Canada balsam. 



(6) Complete rehydration if specimens in aqueous media 

 are desired. 



9. Partial rehydration. 



10. Staining. 



11. Removal of excess stain. 



12. Dehydration. 



13. Clearing. 



14. Mounting in Canada balsam. 



Non-infiltration Method: 



1 . Hardening of soft materials or softening of hard materials. 



2. Blocking. 



3. Sectioning. 



4. Removal of blocking material. 



(a) Staining may be accomplished at this point. 



5. Mounting in glycerin jelly. 



