STAINING 69 



Aniline water safranin stains suberized walls yellowish and 

 lignified walls blue. Allow the stain to act for a least thirty 

 minutes, wash in acid alcohol, then in alcohol, until washings 

 are colorless. 



Gums. 



Mucilaginous cell walls do not stain satisfactorily. 

 Pectins. 



Fuchsin stains pectic substances, lignified tissues and suberin 

 deep red. Upon washing the stained specimens with acid 

 alcohol, the pectic substances are decolorized while lignin 

 and suberin retain the color. The time required is between 

 fifteen and thirty minutes. 



