APPENDIX 



FORMULAE 

 ACID ALCOHOL: 



Cone. Hydrochloric Acid 0.5 mil 



Alcohol, to make 100.0 mils 



ALCOHOL, GLYCERIN, WATER MIXTURE: 



Used as a temporary mounting medium and for maceration of 

 materials. 



Alcohol, 



Glycerin, 



Distilled Water, equal parts by volume. 



ALKANNIN SOLUTION: 

 Formula A 



Alkanct root 20 grams 



Water 100 mils 



Macerate for several days. Filter and dilute with equal parts of 

 water before using. 

 Formula B 



Alkannin dissolved in absolute alcohol. Add equal parts 

 of water and filter before using. 



ALPHA NAPHTHOL: 



Alpha Naphthol 20 grams 



Alcohol, to make 100 mils 



ANILINE DYES: 



Saturated alcoholic solution of dye 5 mils 



Distilled Water, to make 100 mils 



ANILINE WATER SAFRANIN: 



1. Aniline water. 



Saturate distilled water with aniline by vigorous shaking. 



2. Safranin, saturated alcoholic solution. 



Mix equal volumes of 1 and 2 and filter before using. This reagent 

 should be freshly prepared. 



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