APPENDIX 225 



ALTMAN'S PICRIC ACID : 



Picric Acid 8 grams 



Alcohol, to make 100 mils 



Dilute with two volumes of water before using. 



BOTTIN'S FLUID: 



Fixing agent for animal and vegetable materials. 



Picric acid, saturated alcoholic solution 200 mils 



Formalin 80 mils 



Glacial Acetic Acid 15 mils 



Time required is two to five days. After fixing, wash the speci- 

 mens with 50 per cent alcohol until the latter fails to show yellow 

 coloration. 



CHROMIC ACID: 



Chromic Acid 1 gram 



Distilled Water, to make 100 mils 



CHLORAL HYDRATE: 



Chloral Hydrate : 80 grams 



Distilled Water 50 mils 



CLEANING FLUIDS FOR GLASSWARE AND SLIDES: 



Cone. Nitric Acid 1 part 



Cone. Hydrochloric Acid 4 parts 



Uncovered glass containers must be used for this mixture and, 

 owing to the corrosive fumes liberated, vessels containing glassware 

 and cleaning fluid must be kept under a hood or in the open air. After 

 immersion for a few hours the glassware should be well washed in 

 water. 



Potassium dichromate 20 grams 



Water 100 mils 



Cone. Sulphuric Acid 100 mils 



Dissolve the dichromate in the water, add the acid cautiously and 

 in small portions, cooling the mixture between each addition. The 

 mixture must be stored in glass containers and may be repeatedly used 

 until the color changes. Immersion for a few hours followed by washing 

 in water will serve to clean slides and covers. 



