APPENDIX 227 



Copper Half. 



Copper Sulphate 3.4 grams 



Distilled Water, to make 50.0 mils 



Mix equal parts of each solution. 



FERRIC CHLORIDE: 



Ferric Chloride 1.0 gram 



Distilled Water 100 .0 mils 



FIXING FLUIDS: 



Refer to Bouin's Fluid, Chromic Acid, Formalin, Mercuric Chloride, 

 Picric Acid, Picric Sulphuric Acid and Zenker's Fluid. 



FORMALIN: 



Used as a preservative and fixing fluid for animal and vegetable 

 tissues. 



Formalin 10 mils 



Water 90 mils 



Time required for fixing is two to five days. This solution is not 

 always thorough in its fixing action and sometimes interferes with 

 staining. 



GLYCERIN JELLY: 



Gelatin 10 grams 



Glycerin 70 mils 



Water 60 mils 



Liquefied Phenol 1 mil 



Soak the gelatin in water until soft; add the glycerin and gently 



warm until thoroughly dissolved. Add the phenol and filter through 



glass wool. 



IODINE POTASSIUM IODIDE (DILUTE LUGOL'S SOLUTION) : 



Iodine . 35 gram 



Potassium Iodide 1 . 50 grams 



Distilled Water, to make 100 . 00 mils 



MERCURIC POTASSIUM IODIDE (MAYER'REAGENT) : 



Mercuric Chloride 1 . 35 grams 



Potassium Iodide 5.00 grams 



Dissolve the mercuric chloride in 60 mils of distilled water and the 



iodide in 10 mils of distilled water. Mix these solutions and add 



water to make 100 mils. 



