ANATOMY AND HISTOLOGY OF THE OVARIES 541 



suprarenal, they appear to differ from other body fats. Parlion, Dumi- 

 tresco, and Nissipesco ('09) differentiate between them as follows, quoting 

 from Athias: 



Ovarian Lipoid Body Fat 



Toluidin blue Greenish blue 



Sections in Flemming's fluid 



1-5 minutes Brownish vellow 



Sections in acetone 10 min- 

 utes Soluble 



Sections in absolute alcohol 



warmed 10-15 minutes Soluble 



No color 



Black 



Relatively insoluble 



Relatively insoluble 



Mulon ('12) feels that the lipoid is a mixture of phosphatids, fatty 

 acids, and cholesterin. It undoubtedly varies in composition in different 

 animals. It would be well worth while to try to find out whether this 



Fig. 2. Section through the covering of a normal bat's ovarian follicle, i, group 

 of interstitial cells; v, vein; and Ig, lumen formed through the grouping of the inter- 

 stitial cells. After 0. Van der Stricht. 



lipoid differs in any distinctive way, in character or in amount, in animals 

 which Elliot has found to lack doubly refracticle lipoid in their suprarenal 

 glands. Mulon and Mile, de Jong ('13) have made a special study of the 

 chemistry of the lipoid in the thecal cells of human atretic follicles, and 

 have found that it is doubly refractile, stains with scharlach, is not colored 

 or fixed by osmic acid, and gives the reaction of Salkowsky. In the inter- 

 stitial cells the lipoid varies considerably in one and the same cell with 

 respect to the size of the droplets, reaction with osmic acid, and solubility. 

 Athias (e) ('19, p. 195) shares with others the opinion that it is formed 



