486 JACOB ROSEKBLOOM 



however, was able to prove the presence of a substance giving reactions 

 similar to those of Bence-Jones' protein, in the vertebrae and ends of the 

 femur in a case of multiple myeloma, but he could not detect this sub- 

 stance in any other tissue or organ. Bruce, Lund, and Whitcomb found, in 

 a case of multiple myeloma, that the fluid obtained from an affected bone, 

 after sawing through it, gave the reaction of the Bence-Jones' protein. 

 Ribbinik could not find Bence-Jones' protein in the bone-marrow sub- 

 stance of the case studied by him. Fleischer (&), however, has found a sub- 

 stance giving the reaction of Bence-Jones' protein in normal bone-marrow. 

 In a case of Weber's, microscopic section of some of the organs and the 

 new growth in multiple myeloma showed the presence of a homogeneous 

 hyaline substance which he thought might possibly be Bence-Jones' pro- 

 tein. Bradshaw and Warrington, in an analysis of a rib affected with 

 multiple myeloma, found the relation of organic and inorganic substances 

 to be practically normal. 



Digestive Products of Bence-Jones' Protein. Moitessier on subjecting 

 Bence-Jones' protein to gastric digestion obtained metaprotein, primary 

 proteose (except heteroproteose), secondary proteose and peptone. After 

 peptic digestion of Bence-Jones' protein, Simon could not detect primary 

 proteose among the products formed, but found deuteroproteose "B" 

 (Pick) and peptone "A" (Pick). Coriat found Bence-Jones' protein 

 digestible in artificial pancreatic juice. 



General Chemical Nature of Bence-Jones' Protein. Magnus-Levy pub- 

 lished results of a study of the digestive products of Bence-Jones' pro- 

 tein, its reactions and contents of amid, diamino and monoamino nitro- 

 gen. Iluppert recorded results of various elementary analyses that have 

 been made of Bence-Jones' protein. Abderhalden and Rostoski made an 

 analysis of Bence-Jones' protein to determine the amounts of the various 

 amino acids contained. Hopkins and Savory found that Bence-Jones' 

 protein yields all the amino acids characteristic of typical protein, and 

 that it contains a large proportion of aromatic radicals. 



Reach gave the results of an analysis of Bence-Jones' protein in terms 

 of its nitrogen partition. Gross and Allard(a) administered 10 gm. of 

 Bence-Jones' protein to an alkaptonuric subject, with otherwise unaltered 

 diet. There was a large increase in the output of homogentisic acid, 

 which suggested to them that Bence-Jones' protein is rich in aromatic 

 radicals. 



Bence-Jones' Protein in Urine. Zuelzer(6) obtained Bence-Jones' 

 protein in the urine of dogs poisoned with pyrodin (monoacetylphenylhy- 

 drazin), a strong hemolytic agent. Stokvis found Bence-Jones' protein in 

 the urine of dogs after its intravenous or rectal injection. Matthes also 

 found it in the urine of a dog after the subcutaneous injection of Bence- 

 Jones' protein. Ellinger introduced 5 gm. of Bence* Jones' protein in- 

 travenously into a dog, but the urine yielded no precipitate with 



