BENCE-JONES' PROTEIN 487 



(NH 4 ) 2 SO 4 , although the liquid gave a strong biuret reaction suggestive 

 of peptone (Kuehne), which may have been derived from the injected 

 material. I have studied a case in which the Bence-Jones' protein was 

 spontaneously precipitated from the urine. 



Allard and Weber and Decastello have found that the roentgen ray 

 treatment of the bone tumor had no effect on the urinary output of Bence- 

 Jones' protein. Walters has recently found that radium exposure over two 

 femoral masses caused no reduction in the amount of Bence-Jones protein 

 excreted in the 64 days following the exposure. Voit and Salvendi re- 

 ported a case in which diet appeared to modify the elimination of Bence- 

 Jones' protein, but Weber observed that changes of diet had no influence 

 on its elimination in his case of multiple myeloma. Hopkins and Savory 

 found that the amount of excreted Bence-Jones' protein was proportional 

 to the extent of metabolism rather than to any other factor. Massini claimed 

 that the amount of the Bence-Jones' protein excreted was proportional to 

 the amount of the protein of the food. Walters found that the quantity of 

 Bence-Jones' protein excreted is independent of the protein intake and the 

 amount of the protein excreted during the night when food is not taken is 

 only slightly less than the amount excreted during the day. He claims 

 that there is not a constant relationship between the amount of Bence- 

 Jones' protein excreted and the total urinary nitrogen excreted. 



Bence-Jones' Protein in Blood and Lymph. Ribbinik and Askanazy 

 could not find Bence-Jones' protein in the blood of patients with multiple 

 myeloma. Ellinger obtained it from ascitic fluid. Coriat found Bence- 

 Jones' protein in a pleural effusion from a patient suffering from multiple 

 neuritis associated with extreme tenderness of the ribs, although the pro- 

 tein was absent from the urine. Rostoski found that the method of "pre- 

 cipitin" detection fails to distinguish Bence-Jones' protein from various 

 proteins of human origin. Donati and Satta have shown that serum albu- 

 min, serum globulin, edestin, egg albumin, milk and milk serum, inhibit 

 the hemolytic action of sodium glycocholate and sodium oleate, whereas 

 ovo-albumin, Bence-Jones' protein and casein accelerate the hemolysis. 

 Borchart and Lippmann found that after feeding Bence-Jones' protein to 

 fasting dogs, it could be detected in serum from the animals by the pre- 

 cipitin test and in samples of their blood by chemical tests. Abderhalden 

 has lately isolated the protein from the blood of a patient with multiple 

 myeloma. 



II. Proteosuria and Bence-Jones' Protein 



Proteoses have been found in the urine under many conditions, usually 

 in minute quantities and as temporary constituents of the urine during 

 the course of specific fevers, inflammatory processes, and other diseases. 

 The urinary proteoses present different characteristics from those of 



