584 



SAMUEL H. HURWITZ 



output of ammonia was observed in these conditions, but this may be partly 

 the result of such factors as inanition, and not therefore directly at- 

 tributable to the anemia. 



Observations on the nitrogen partition in chlorosis are not numerous. 

 These have been analyzed by Vannini in his careful metabolic study of 

 this disease. Great fluctuations in the percentage of urea and ammonia 

 nitrogen are recorded by most workers. For urea the values found range 

 from 83 to 92 per cent, and for ammonia from about 2 to 9.5 per cent 

 of the total nitrogen. Although Vannini's figures are in accord with those 



Fig. 3. Effect of hemorrhage on the excretion of creatinin, creatin, uric acid and 

 total nitrogen in the pig. The vertical lines represent the averages of the actual 

 amounts excreted during various periods of the experiment. Line I represents the 

 fore-period, Line II the period between the first and second hemorrhages, Line III the 

 period between the second and third hemorrhages. (After Buell, M.V., J. Biol. Chem., 

 1919, xl, 75.) 



of earlier observers so far as the percentage of urea nitrogen is con- 

 cerned, his ammonia percentages are considerably lower than the average, 

 ranging from 2.06 to 2.63 per cent. 



Determinations by different workers of the various urinary nitrogen 

 fractions in the Tiemolytic anemias are also lacking in uniformity. In a 

 careful metabolic study of experimental pyrodin anemia in animals, 

 Samuely(a) noted that as the anemia progressed the urea percentage 

 fell from 86.3 to 72.14 per cent, whereas the ammonia and the amino-acid 

 fractions increased. 



In patients with pernicious anemia, a change in the partition of the 

 nitrogenous substances in the urine has been observed only in exceptional 

 cases, Minot(a) found a low urea, percentage and a normal ammonia out- 



